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human serum depleted of individual complement components (c3, factor b and factor d)  (Complement Technology Inc)

 
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    Complement Technology Inc human serum depleted of individual complement components (c3, factor b and factor d)
    iC3b:plasma protein complexes bind to red blood cells (RBCs). Isolated human RBCs were treated for 30 minutes at 37°C with either with sham-treated NHS or serum activated with <t>CVF.</t> Serum aliquots were removed before (pre-RBC Tx) and after (post-RBC Tx) RBC treatment. RBCs were lysed with H2O and the membrane fraction (pellet) was separated from the supernatant. All samples were separated using SDS-PAGE on an 8% gel and immunoblotted <t>for</t> <t>C3.</t>
    Human Serum Depleted Of Individual Complement Components (C3, Factor B And Factor D), supplied by Complement Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+serum+depleted+of+individual+complement+components+(c3%2C+factor+b+and+factor+d)/complement+c9+depleted+human+serum++c9d+nhs/pmc04282830-43-3-35
    Average 90 stars, based on 1 article reviews
    human serum depleted of individual complement components (c3, factor b and factor d) - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "Enhanced recognition of plasma proteins in a non-native state by complement C3b. A possible clearance mechanism for damaged proteins in blood"

    Article Title: Enhanced recognition of plasma proteins in a non-native state by complement C3b. A possible clearance mechanism for damaged proteins in blood

    Journal: Molecular immunology

    doi: 10.1016/j.molimm.2014.10.022

    iC3b:plasma protein complexes bind to red blood cells (RBCs). Isolated human RBCs were treated for 30 minutes at 37°C with either with sham-treated NHS or serum activated with CVF. Serum aliquots were removed before (pre-RBC Tx) and after (post-RBC Tx) RBC treatment. RBCs were lysed with H2O and the membrane fraction (pellet) was separated from the supernatant. All samples were separated using SDS-PAGE on an 8% gel and immunoblotted for C3.
    Figure Legend Snippet: iC3b:plasma protein complexes bind to red blood cells (RBCs). Isolated human RBCs were treated for 30 minutes at 37°C with either with sham-treated NHS or serum activated with CVF. Serum aliquots were removed before (pre-RBC Tx) and after (post-RBC Tx) RBC treatment. RBCs were lysed with H2O and the membrane fraction (pellet) was separated from the supernatant. All samples were separated using SDS-PAGE on an 8% gel and immunoblotted for C3.

    Techniques Used: Clinical Proteomics, Isolation, Membrane, SDS Page

    Thermally denatured proteins activate the complement system via the alternative pathway. NHS was heated to 60°C for 1 hour (Δ serum) and then added to equal volume of either fresh NHS (lanes 2), factor B depleted serum (lanes 5), factor D depleted serum (lanes 6) or C3 depleted serum (lanes 7) and incubated for either 15 or 60 minutes at 37°C. The negative control was untreated NHS (lanes 1) or NHS that received equal amount of Δ serum in the presence of 10 mM EDTA (lanes 3) to prevent complement activation. The positive control was a mixture of Δ serum plus fresh serum treated with CVF (lanes 4) to activate the alternative pathway. Samples were separated using an 8% SDS-PAGE gel and blotted for factor B (alternative pathway activation and generation of the 33 kDa Ba fragment), C3 (110 kDa α-chain cleavage and 67 kDa iC3b generation) and α1PI (iC3b:α1PI complex formation).
    Figure Legend Snippet: Thermally denatured proteins activate the complement system via the alternative pathway. NHS was heated to 60°C for 1 hour (Δ serum) and then added to equal volume of either fresh NHS (lanes 2), factor B depleted serum (lanes 5), factor D depleted serum (lanes 6) or C3 depleted serum (lanes 7) and incubated for either 15 or 60 minutes at 37°C. The negative control was untreated NHS (lanes 1) or NHS that received equal amount of Δ serum in the presence of 10 mM EDTA (lanes 3) to prevent complement activation. The positive control was a mixture of Δ serum plus fresh serum treated with CVF (lanes 4) to activate the alternative pathway. Samples were separated using an 8% SDS-PAGE gel and blotted for factor B (alternative pathway activation and generation of the 33 kDa Ba fragment), C3 (110 kDa α-chain cleavage and 67 kDa iC3b generation) and α1PI (iC3b:α1PI complex formation).

    Techniques Used: Incubation, Negative Control, Activation Assay, Positive Control, SDS Page

    C3 dependent complement activators generate C5a in thermally denatured serum. Untreated NHS (Control) or NHS plus 60°C heated serum (Δ Serum) were treated with either CVF, zymosan, HAGG or buffer (Sham) for 60 minutes at 37°C and C5a levels were quantified by ELISA.
    Figure Legend Snippet: C3 dependent complement activators generate C5a in thermally denatured serum. Untreated NHS (Control) or NHS plus 60°C heated serum (Δ Serum) were treated with either CVF, zymosan, HAGG or buffer (Sham) for 60 minutes at 37°C and C5a levels were quantified by ELISA.

    Techniques Used: Control, Enzyme-linked Immunosorbent Assay



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    human serum depleted of individual complement components (c3, factor b and factor d)  (Complement Technology Inc)
    90
    Complement Technology Inc human serum depleted of individual complement components (c3, factor b and factor d)
    iC3b:plasma protein complexes bind to red blood cells (RBCs). Isolated human RBCs were treated for 30 minutes at 37°C with either with sham-treated NHS or serum activated with <t>CVF.</t> Serum aliquots were removed before (pre-RBC Tx) and after (post-RBC Tx) RBC treatment. RBCs were lysed with H2O and the membrane fraction (pellet) was separated from the supernatant. All samples were separated using SDS-PAGE on an 8% gel and immunoblotted <t>for</t> <t>C3.</t>
    Human Serum Depleted Of Individual Complement Components (C3, Factor B And Factor D), supplied by Complement Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+serum+depleted+of+individual+complement+components+(c3%2C+factor+b+and+factor+d)/complement+c9+depleted+human+serum++c9d+nhs/pmc04282830-43-3-35
    Average 90 stars, based on 1 article reviews
    human serum depleted of individual complement components (c3, factor b and factor d) - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

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    iC3b:plasma protein complexes bind to red blood cells (RBCs). Isolated human RBCs were treated for 30 minutes at 37°C with either with sham-treated NHS or serum activated with CVF. Serum aliquots were removed before (pre-RBC Tx) and after (post-RBC Tx) RBC treatment. RBCs were lysed with H2O and the membrane fraction (pellet) was separated from the supernatant. All samples were separated using SDS-PAGE on an 8% gel and immunoblotted for C3.

    Journal: Molecular immunology

    Article Title: Enhanced recognition of plasma proteins in a non-native state by complement C3b. A possible clearance mechanism for damaged proteins in blood

    doi: 10.1016/j.molimm.2014.10.022

    Figure Lengend Snippet: iC3b:plasma protein complexes bind to red blood cells (RBCs). Isolated human RBCs were treated for 30 minutes at 37°C with either with sham-treated NHS or serum activated with CVF. Serum aliquots were removed before (pre-RBC Tx) and after (post-RBC Tx) RBC treatment. RBCs were lysed with H2O and the membrane fraction (pellet) was separated from the supernatant. All samples were separated using SDS-PAGE on an 8% gel and immunoblotted for C3.

    Article Snippet: 2.1 Reagents Purified cobra venom factor (CVF), human serum depleted of individual complement components (C3, factor B and factor D), and purified human complement proteins (C3, factor B and factor D) were all purchased from Complement Technology, Inc. (Tyler, TX).

    Techniques: Clinical Proteomics, Isolation, Membrane, SDS Page

    Thermally denatured proteins activate the complement system via the alternative pathway. NHS was heated to 60°C for 1 hour (Δ serum) and then added to equal volume of either fresh NHS (lanes 2), factor B depleted serum (lanes 5), factor D depleted serum (lanes 6) or C3 depleted serum (lanes 7) and incubated for either 15 or 60 minutes at 37°C. The negative control was untreated NHS (lanes 1) or NHS that received equal amount of Δ serum in the presence of 10 mM EDTA (lanes 3) to prevent complement activation. The positive control was a mixture of Δ serum plus fresh serum treated with CVF (lanes 4) to activate the alternative pathway. Samples were separated using an 8% SDS-PAGE gel and blotted for factor B (alternative pathway activation and generation of the 33 kDa Ba fragment), C3 (110 kDa α-chain cleavage and 67 kDa iC3b generation) and α1PI (iC3b:α1PI complex formation).

    Journal: Molecular immunology

    Article Title: Enhanced recognition of plasma proteins in a non-native state by complement C3b. A possible clearance mechanism for damaged proteins in blood

    doi: 10.1016/j.molimm.2014.10.022

    Figure Lengend Snippet: Thermally denatured proteins activate the complement system via the alternative pathway. NHS was heated to 60°C for 1 hour (Δ serum) and then added to equal volume of either fresh NHS (lanes 2), factor B depleted serum (lanes 5), factor D depleted serum (lanes 6) or C3 depleted serum (lanes 7) and incubated for either 15 or 60 minutes at 37°C. The negative control was untreated NHS (lanes 1) or NHS that received equal amount of Δ serum in the presence of 10 mM EDTA (lanes 3) to prevent complement activation. The positive control was a mixture of Δ serum plus fresh serum treated with CVF (lanes 4) to activate the alternative pathway. Samples were separated using an 8% SDS-PAGE gel and blotted for factor B (alternative pathway activation and generation of the 33 kDa Ba fragment), C3 (110 kDa α-chain cleavage and 67 kDa iC3b generation) and α1PI (iC3b:α1PI complex formation).

    Article Snippet: 2.1 Reagents Purified cobra venom factor (CVF), human serum depleted of individual complement components (C3, factor B and factor D), and purified human complement proteins (C3, factor B and factor D) were all purchased from Complement Technology, Inc. (Tyler, TX).

    Techniques: Incubation, Negative Control, Activation Assay, Positive Control, SDS Page

    C3 dependent complement activators generate C5a in thermally denatured serum. Untreated NHS (Control) or NHS plus 60°C heated serum (Δ Serum) were treated with either CVF, zymosan, HAGG or buffer (Sham) for 60 minutes at 37°C and C5a levels were quantified by ELISA.

    Journal: Molecular immunology

    Article Title: Enhanced recognition of plasma proteins in a non-native state by complement C3b. A possible clearance mechanism for damaged proteins in blood

    doi: 10.1016/j.molimm.2014.10.022

    Figure Lengend Snippet: C3 dependent complement activators generate C5a in thermally denatured serum. Untreated NHS (Control) or NHS plus 60°C heated serum (Δ Serum) were treated with either CVF, zymosan, HAGG or buffer (Sham) for 60 minutes at 37°C and C5a levels were quantified by ELISA.

    Article Snippet: 2.1 Reagents Purified cobra venom factor (CVF), human serum depleted of individual complement components (C3, factor B and factor D), and purified human complement proteins (C3, factor B and factor D) were all purchased from Complement Technology, Inc. (Tyler, TX).

    Techniques: Control, Enzyme-linked Immunosorbent Assay